Terminator

Part:BBa_K1021008:Design

Designed by: Swati Sureka   Group: iGEM13_Cornell   (2013-09-13)


TtrpC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 485
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 201


Design Notes

The terminator is best used in conjunction with PtrpC (BBa_K1021007).


Source

Fungal transformation vector pUCATPH (http://www.snapgene.com/resources/plasmid_files/yeast_plasmids/pUCATPH/), described in http://www.pnas.org/content/91/26/12649.full.pdf.

References